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13) L1G0 refers to the conditions Po and T0 The pressure dependence can be taken into account starting from the volume change that is not only pressure dependent but also temperature dependent: aV(p,T)avaa(T-T0 )-af3(p-p0 ). The low molecular weight analytes are separated across small membrane (cellulose acetate, cut-off 1000- 60 000 Da) windows into a Sowing acceptor stream, as shown in Figure 5B. Low- speed centrifugation thus facilitates the easy and selective collection of inclusion bodies directly after cellular homogenization.
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